In terms of influencing factors, grade, gender, parents' education and knowledge sources all have an impact on health literacy. In terms of basic knowledge, concepts and basic skills, the health literacy level of senior students is higher than that of junior middle school students. In addition, the awareness rates of senior medical students in infectious disease prevention, chronic disease prevention, safety and first aid, and basic medical care are higher than that of junior medical students, indicating that classroom education plays an important role in the following aspects. To improve the health literacy of medical students. Health literacy level is closely related to health education [9]. Female students have a higher level of healthy lifestyle and behavioral health literacy than male students, which may be related to the fact that female students pay more attention to the accumulation of health knowledge and their own image. Medical students with higher education level and those with lower education level had statistical significance in the availability of basic health knowledge and concepts, indicating that family education and family environment have certain influence on college students' health literacy level.