Add 1 mL of the prepared broth to 2-10 tubes. No.1 test tube, without adding broth medium, aspirate 2 mL of preparation drug using a pipettePour the liquid (Honeysuckle Dew) into Test Tube # 1. Put 1 mL of the solution of test tube No. 1 into test tube No. 2, shake and mix in test tube No. 2, and mix well.Using a pipette, remove 1 mL of the diluted solution from Test Tube No. 2, add it to Test Tube No. 3, and perform the same operation from Test Tube No. 4 to Test Tube No. 10. Next, start the No. 10 test tube.Aspirate 1 mL of solution from the tube and drain into the sewer. Test tube No. 11 is used as a control test tube without adding chemicals. Add 0.1 mL of the prepared bacterial solution to each test tube and addAfter mixing all the tubes uniformly, place them in a constant temperature incubator at 36 ° C for 1 day.