MicroRNAs在细胞应激调节反应中起着重要的作用。我们之前利用So

MicroRNAs play important roles in regulating cellular stress reactions. Before we take advantage of the depth of Solexa sequencing technology found in 10 kinds of x-ray-induced new human mirna in HeLa cells. Wherein, miR-5094, is predicted to be targeted STAT5b. This study aimed to explore whether miR-5094 by STAT5b involved in cell radiation response. First, it was confirmed miRNA-5094 stat5b3'-UTR and through direct interactions with luciferase reporter gene experiments. Then, STAT5b downstream gene expression is detected after ionizing radiation (IR) in HeLa cells. Finally, we have found that by radiation-induced or mock infected miRNA modulation STAT5b inhibit the expression of miR-5094, thereby reducing the transcription of downstream Igf-1 and Bcl-2's. In addition, miR-5094 is low and the knock miRNA inhibitor inhibits, partially reversed the miR-5094 in HeLa cells, Jurkat cells and CD4 + T cell proliferation induced. In conclusion, our results indicate that, miR-5094 is down regulated STAT5b expression, thereby inhibiting the proliferation of cells after the x-ray radiation.

MicroRNAs play an important role in cellular stress regulation. We previously discovered 10 new x-ray-induced human mirnas in HeLa cells using Solexa deep sequencing techniques. Among them, miR-5094, is predicted to target STAT5b. The purpose of this study is to explore whether miR-5094 is involved in cellular radiation response through STAT5b. First, gene experiments with fluorerase reporting confirmed that miRNA-5094 has direct interaction with stat5b3'-UTR. The expression of the downstream STAT5b gene after ionizing radiation (IR) is then detected in HeLa cells. Finally, we found that the expression of STAT5b can be inhibited by radiation-induced or miRNA analog transduction tomodulate mi-5094, thereby reducing the transcription of downstream Igf-1 and Bcl-2. In addition, miR-5094 was inhibited and knocked down by miRNA inhibitors, partially reversing the proliferation inhibition induced by miR-5094 in HeLa cells, Jurkat cells, and CD4-T cells. In summary, our results show that the upward adjustment of miR-5094 lowers the expression of STAT5b, thus inhibiting the proliferation of cells after x-ray exposure.

MicroRNAs play an important role in cell stress regulation. We have previously found 10 new human miRNAs induced by X-ray in HeLa cells by Solexa deep sequencing technology. Among them, mir-5094 is predicted to target STAT5b. The purpose of this study is to explore whether mir-5094 participates in cell radiation response through STAT5b. First, the direct interaction between mirna-5094 and stat5b3 '- UTR was confirmed by luciferase reporter gene experiment. Then, the expression of STAT5b downstream gene after ionizing radiation (IR) was detected in HeLa cells. Finally, we found that up regulation of mir-5094 by radiation-induced or miRNA mimic transfection could inhibit the expression of STAT5b, thus reducing the transcription of downstream IGF-1 and bcl-2. In addition, mir-5094 was inhibited and knocked down by miRNA inhibitors, which partially reversed the proliferation inhibition induced by mir-5094 in HeLa cells, Jurkat cells and CD4 + T cells. In conclusion, our results showed that the up-regulation of mir-5094 down regulated the expression of STAT5b, thus inhibiting the proliferation of cells after X-ray irradiation.