b.Transformationi.Confirm mutants using PCR, restriction digests (as appropriate) and sequencing4.Clean one is interesting so investigate E98R furthera.Fluorescence +/- DMBQ & DCMU (assembly info)5.Include your verification of the ∆psb27 strains – include diagram of how they were constructed. Confirm if clean or not. Describe why you selected the version you used for new transformationsⅣ. Discussion1.Biological significance of the introduced changes in Psb27a.O2 all similar to control except E98R with DMBQi.suggest acceptor effect changing efficiency of DMBQb.Discuss – what the fluorescence shows in the presence and absence of DMBQ 2.Technical significancea.Constructed clean strains for further studyⅤ. Reference