Experimental set-up for the isolation of dermal fibroblasts. After tissue digestion, FACS was used to select cells that were negative for the lineage markers CD31 (endothelial marker), CD45 (immune cell marker), CD24 (pre-adipocyte marker), EpCAM (epithelial marker), and CD117 (melanocyte marker), and positivefor the fibroblast markers Pdgfra and CD34.