Lipid extract for sample and lipid weight determination. For thelipid extraction, 15 g of homogenized dried sample were ground topowder, and extracted with 200 mL acetone/n-hexane (2:1 v/v) for8 min with stirred and filtration, repeat this procedure again with200 mL acetone/hexane solution, afterword, the extract was placedin a 60 °C water bath to decompress and concentrate to dry, and thecontent of lipid that performed on an aliquot of the exact, could bedetermined by weight method.