After PCR with EvaGreen® Plus Dye, PCR products do not need to be stained with another DNA gel stain for gel electrophoresis. Simply add DNA loading buffer to your PCR reaction solution, load on a gel, and conduct electrophoresis as usual. Gel visualization can be carried out using a 254 nm UV box, or a blue LED imager using a SYBR® Green filter. Alternatively, the gel may be imaged using a 488 nm laser-based gel scanner.