With the rise of modern farming mode, more and more attention has been paid to the rational use of antibiotics in animal husbandry. Antibiotics can be used to treat and prevent diseases, increase feed conversion and promote animal growth. Due to the long-term, extensive and unreasonable use of antibiotics and the selection pressure of antibiotics, the number of resistant strains of e. coli increased, and the spectrum of resistance increased, resulting in the emergence of multi-drug resistant strains, which brought serious problems to the prevention and treatment of diseases. Under the selection pressure, antimicrobial drugs will develop drug resistance, resulting in an increase in the species and number of drug-resistant bacteria and the ability to pass drug-resistant genes to their offspring, thus forming a "drug-resistant bacteria bank". Therefore, how to reduce bacterial resistance has become the focus of scientists. In combination with the actual development of aquaculture, rational drug use is one of the most appropriate methods. The active research on the resistance of escherichia coli is helpful to prevent the emergence and development of the resistance of escherichia coli and promote the use of antibiotics more rational. 本文对多抗性猪源大肠杆菌的分离鉴定及耐药性检测进行了详细分析。