Bacterial wilt is a highly destruct

Bacterial wilt is a highly destructive bacterial soil-borne vascular disease caused by Ralstonia solanacearum. This fungus has strong habituation and can survive in the soil and on non-host plants for a long time. At the same time, R. solanacearum has genetic diversity and is a compound species. The fungus has obvious physiological differentiation and high variability in the process of co-evolution with the host in different geographical origins. Therefore, the control of R. solanacearum has always been a difficult problem. . At present, most of the control methods for bacterial wilt are the use of disease-resistant varieties and chemical pesticide control, but the resistance of disease-resistant varieties will gradually weaken or even lose with each generation, and chemical control will also bring serious damage to the environment and endanger human health. Biological control is a means of using organisms and their metabolites to prevent and control plant diseases. Microbial control has the characteristics of no drug resistance, environment and human friendliness, making it gradually become a popular method for the prevention and treatment of bacterial wilt. On the basis of isolating and screening the rhizosphere microorganism Pseudomonas mosselii that inhibits bacterial wilt, this study predicted the synthesis of gene cluster of its bacteriostatic products through bioinformatics through reverse genetics methods, constructed deletion and complement mutants, and identified the role of P. mosselii active substance Pseudopyroninein resistance to bacterial wilt, The aim is to determine the effectiveness of P. mosselii in preventing and controlling bacterial wilt of crops such as nightshade in production.