Cyperus rotundus Linn. rhizomes extracts were evaluated against six important pathogenic microbes viz. Staphylococcus epidermidis, Bacillus cereus, Escherichia coli, Aspergillus niger and Candida albicans . The powdered rhizome extracts were successively extracted with petroleum ether, chloroform, ethanol and water using Soxhlet apparatus. The antibacterial and antifungal activities were performed by both agar well diffusion and serial dilution methods. The ethanolic extract was found to exhibit highest activity against tested bacteria. However all extracts were ineffective against fungal strains. The inhibitory effect is very similar and comparable with that of standard drug.