根据紫杉醇的结构特点和红豆杉中紫杉醇的合成机制,选取了几种前体物质,研

根据紫杉醇的结构特点和红豆杉中紫杉醇的合成机制,选取了几种前体物质,研究其对红豆杉内生真菌合成紫杉醇的影响。结果表明,在发酵培养的第10天,补加下列任一前体物质,使发酵液中初始浓度分别达到苯甲酸钠30.0mg/L,酪氨酸20.0mg/L,L-苯丙氨酸3.0mg/L,乙酸钠3.0g/L,均能提高紫杉醇产量。进一步采用L16(45)正交试验分析得到,4种前体物质在培养基中的最佳组合为A3B3C3D1,即L-苯丙氨酸3.0mg/L,酪氨酸40.0mg/L,乙酸钠3.0g/L,苯甲酸钠10.0mg/L,对应发酵液中紫杉醇含量达到987.3μg/L。

根据紫杉醇的结构特点和红豆杉中紫杉醇的合成机制,选取了几种前体物质,研究其对红豆杉内生真菌合成紫杉醇的影响。 结果表明,在发酵培养的第10天,补加下列任一前体物质,使发酵液中初始浓度分别达到苯甲酸钠30.0mg/L,酪氨酸20.0mg/L,L-苯丙氨酸3.0mg/L,乙酸钠3.0g/L,均能提高紫杉醇产量。 进一步采用L16(45)正交试验分析得到,4种前体物质在培养基中的最佳组合为A3B3C3D1,即L-苯丙氨酸3.0mg/L,酪氨酸40.0mg/L,乙酸钠3.0g/L,苯甲酸钠10.0mg/L,对应发酵液中紫杉醇含量达到987.3μg/L。

According to the structural characteristics of taxol and the synthesis mechanism of taxol in Taxus, several precursors were selected to study their effects on the synthesis of taxol by endophytic fungi of Taxus. The results showed that on the 10th day of fermentation, the initial concentration of sodium benzoate, tyrosine, L-phenylalanine and sodium acetate were 30.0mg/l, 20.0mg/l, 3.0mg/l and 3.0g/l, respectively. The results of L16 (45) orthogonal test showed that the best combination of the four precursors in the medium was a3b3c3d1, i.e. L-phenylalanine 3.0mg/l, tyrosine 40.0mg/l, sodium acetate 3.0g/l, sodium benzoate 10.0mg/l. The corresponding taxol content in the fermentation broth reached 987.3 μ g / L.<br>