YFP和GFP其实是序列基本相同的两种蛋白，YFP就是把Thr203以

YFP和GFP其实是序列基本相同的两种蛋白，YFP就是把Thr203以Tyr取代, GFP则不发出绿色荧光，而发出较长波长的黄色荧光，也就是YFP。 因此两者最大的区别则是发射波长了。其最大激发波长为514 nm,最大发射波长为527 nm。

YFP和GFP其实是序列基本相同的两种蛋白，YFP就是把Thr203以Tyr取代, GFP则不发出绿色荧光，而发出较长波长的黄色荧光，也就是YFP。 因此两者最大的区别则是发射波长了。 其最大激发波长为514 nm,最大发射波长为527 nm。

In fact, YFP and GFP are two kinds of proteins with the same sequence. YFP is to replace thr203 with Tyr, while GFP does not emit green fluorescence, but emit longer wavelength yellow fluorescence, that is, YFP. So the biggest difference between the two is the emission wavelength. The maximum excitation wavelength is 514 nm and the maximum emission wavelength is 527 nm.<br>