PS concentration was analyzed using the Dubois method [25]. 200 μl of sample, 200 μl of phenolic solution (5%), and 1 ml of concentrated H2SO4 were mixed and taken to a water bath at 100°C for 5 min. After that, samples were allowed to cool for 30 min in the dark and then photo-metrically analyzed at 485 nm. Results were determined by using a calibration curve prepared with glucose solution