Solvent mixture ace tonitrie R1, wa

Solvent mixture ace tonitrie R1, water for chomatography R (50: 50 V).Test solution Dissolve 40.0 mg of the substance to be examined in the solvent mature and dilute to 50.0 mL with the solvent mixtureReference solution(a) Dissolve 40.0 mg of aprepitant CRS in the solvent mixture and dilute to 50.0 mL with the solent mixture.Reference solution(b) Dilute 1.0 mL of the test solution to 100.0 mL with the solent mixture. Dilute 1.0 mL of this solution to 20.0 mL with the solventmixtureReference solution(c) Dissolve 4 mg of aprepitant for system suitability CRS (containing impurity A)in 5.0 mL of the solent mixture.Column.size:I=0.25 m 0=4.6 mmstationary phase: end-capped octadecylsilyl slica gel for chromatography compatible with 100 per cent aqueous mobile phases R(5 um);temperature:35°CMobile phasemobile phase A: 0.1 per cent V/ solution of phosphoric acid R:mobile phase B: acetonitrile R1TimeM。