细菌传代步骤：1. 第一天，将细菌划线接种于固体琼脂培养基，37°C培

Bacterial subculture steps: <br>1. The first day, the bacteria were streaked on solid agar medium, 37 ° C incubator overnight culture; <br>2. The following day, remove Individual colonies were picked and inoculated in a liquid medium, 37 ° C shaker overnight culture; <br>3. the third day, removed and stored in 20% glycerol concentration, -80 ° C storage.

Bacterial generation steps:<br>1. On the first day, the bacteria are inoculated in solid agar medium, 37 degrees C incubator, and cultured overnight;<br>2. The next day, remove and pick a single clone, inoculated in a liquid medium, 37 degrees C shaker, overnight culture;<br>3. On the third day, remove and store in 20% of the glycerin, and save at -80 degrees C.

Bacterial passage steps:<br>1. On the first day, the bacteria were inoculated on solid agar medium in 37 ° C incubator and cultured overnight;<br>2. On the second day, take out and pick out a single clone, inoculate it in liquid medium, shake it at 37 ° C, and culture overnight;<br>3. On the third day, take it out and store it in 20% glycerol at - 80 ° C.<br>