The whole plant of G. biloba can be used as medicine. In its root, stem, leaf, seed and bark of Ginkgo biloba, various endophytes have been isolated and their biological function was investigated. The conventional procedure of endophytes isolation is to wash the roots, stems or leaves of ginkgo firstly with 75% alcohol for 3 min, rinse with sterile water 3–5 times, 0.1% mercury sterilized for 2 min, rinsed with sterile water 3–5 times, cut into 0.5 cm × 0.5 cm pieces. The cutting pieces were inoculated in PDA medium at 28 °C for 4 days. After purification, ginkgo endophytes were isolated.