立刻塞上塞子，将10ml具塞试管用同一种手法颠倒3次，使试管内壁完全润

Immediately plug the stopper, invert the 10ml stoppered test tube three times with the same method to completely wet the inner wall of the test tube without generating air bubbles. . Note: After adding plasma, it must be placed in an electric heating constant temperature water bath within 20 minutes, and the delay in placing it will affect the experimental results.

Immediately plug the plug and reverse the 10ml plugged test tube for three times with the same method to completely wet the inner wall of the test tube without bubbles. After shaking well, place it in a constant temperature water tank at 37.0 ℃± 1.0 ℃, and the water bath time is 60min ± 1min. Note: after adding plasma, it must be put into the electric heating constant temperature water bath within 20 minutes. The delay will affect the experimental results.

Plug the plug immediately, turn the 10ml test tube with plug upside down three times by the same method, so that the inner wall of the test tube is completely wetted, and no bubbles are generated. Shake well and place it in a constant temperature water tank at 37.0℃ 1.0℃ for 60min±1min. Note: After the plasma is added, it must be put into the electrothermal constant-temperature water bath within 20 minutes. Delaying the introduction will affect the experimental results.