Blood samples were allowed to stand at room temperature for 1 hand then centrifuged at 12000g for 20 min at 4 °C. The supernatant wasaspirated and stored at −80 °C, the total cholesterol (TC), low densitylipoprotein (LDL-C), aspartate aminotransferase (AST) and alanineaminotransferase (ALT) were measured using a UniCel DxC 800Synchron Clinical System (Beckman Coulter, Pasadena, CA, USA).