The high pressure steam sterilizer uses the steam sterilization process under certain pressure. The water in the sterilizer is heated to produce steam. When the cold air is expelled from the sterilizer by Water Vapor, the exhaust valve is closed and the heating continues. The internal pressure of the sterilizer increases gradually, and the boiling point is higher than 100 °C. The microbial protein will coagulate and denature in the high temperature environment, thus the aim of sterilizing [3] is achieved. According to shape and size can be divided into horizontal autoclave, vertical autoclave, portable autoclave, etc. [4] . The most common type used by aseptic medical equipment manufacturers is the vertical high pressure steam sterilizer, mainly used for medical equipment, glassware, other laboratory equipment that can be sterilized by moist heat, eluent, culture medium, discarded objects, moist heat sterilization of bacteria, aseptic clothing, etc. , therefore, its sterilization effect directly affects the authenticity and reliability of the experimental results of microbial detection. It is necessary to develop a plan to periodically validate the effectiveness of the vertical autoclave sterilization process under specified temperature and time conditions.