利用大量已发表的DNA/RNA序列，如EST序列，获得了该物种的微卫星

利用大量已发表的DNA/RNA序列，如EST序列，获得了该物种的微卫星DNA序列。结果表明，EST包含丰富的微卫星序列（约2%-4%），有的种类甚至达到10%以上（如鲶鱼）。Serapion等人。从河鲶（ichthyurus punctatus）公布的43033个EST序列中筛选出4855个含有微卫星的片段。Zhan等人。从海湾扇贝EST文库中成功筛选出19个多态性微卫星标记。大量实验表明，利用公共数据库中的数据进行二次利用是获得新的微卫星标记的一种快速、有效、廉价的方法。

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结果 (英语) 1: [复制]

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Using a large number of published DNA / RNA sequences, such as EST sequences, microsatellite DNA sequences of this species were obtained. The results show that EST contains abundant microsatellite sequences (about 2% -4%), and some species even reach more than 10% (such as catfish). Serapion et al. From the 43,033 EST sequences published by catfish (ichthyurus punctatus), 4,855 fragments containing microsatellites were screened. Zhan et al. 19 polymorphic microsatellite markers were successfully screened from the EST library of scallop scallops. A large number of experiments have shown that using data in public databases for secondary use is a fast, effective, and inexpensive method for obtaining new microsatellite marks.

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结果 (英语) 2:[复制]

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Microsatellite DNA sequences for the species were obtained using a large number of published DNA/RNA sequences, such as EST sequences. The results show that EST contains a rich microsatellite sequence (about 2%-4%), with some species even reaching more than 10% (e.g. mackerel). Serapion et al. 4,855 fragments containing microsatellites were selected from 43,033 EST sequences published by ichthyurus punctatus. Zhan et al. Nineteen polymorphic microsatellite markers were successfully screened from the Bay ScallopEST Library. A large number of experiments show that the use of data in public databases for secondary utilization is a fast, effective and inexpensive method to obtain new microsatellite markers.

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结果 (英语) 3:[复制]

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Using a large number of published DNA / RNA sequences, such as EST sequences, the microsatellite DNA sequences of the species were obtained. The results show that EST contains abundant microsatellite sequences (about 2% - 4%), and some species even reach more than 10% (such as catfish). Serapion et al. 4855 fragments containing microsatellites were screened from 43033 EST sequences published by ichthyurus punctatus. Zhan et al. 19 polymorphic microsatellite markers were successfully screened from EST Library of bay scallop. A large number of experiments show that using the data in the public database for secondary utilization is a fast, effective and cheap way to obtain new microsatellite markers.<br>

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