A published genome-wide association

A published genome-wide association of human gut microbiome was used in this study[29]. In simple terms, the gut microbiota associated SNPs was detected in faecal 16S ribosomal RNA gene sequences and host genotype data from the Flemish Gut Flora Project (n = 2,223) and two German cohorts (n = 1667). Faecal samples from 2,482 FGFP volunteers were sequenced, microbiome data processing, trait preparation, genotyping, and variation quality control. After taking into account the missing data among covariates, 2,223 remained. All microbiome α-diversity, AB and P/A and association analyses were performed using snptest.2.5.0[38]. The FoCus11[39] and PopGen16[40] cohorts were genotyped using the Illumina Omni Express + Exome array and the Affymetrix Genome-Wide Human SNP Array 6.0, respectively. In our study, we selected microbial traits associated SNPs with significant threshold of p < 5×10-6 to calculate PRSs. A detailed description of the subject, experimental design and gene expression analysis can be found in this article