2.7. Induction of HO-1 and GCLC Exp

2.7. Induction of HO-1 and GCLC Expression Is Responsible for the Antioxidant Activities of 1 and 1dTo investigate whether the increased expression of HO-1 and GCLC caused by the identified lead compounds 1 and 1d is responsible for the cytoprotective effects against H2O2-derived oxidative cell death, specific inhibitors, ZnPP and BSO were utilized in this study. We treated ZnPP (15 μM) or BSO (10 μM) in PC12 cells 1 h before the addition of chalcones. Meanwhile, we also determined whether the concentrations of ZnPP and BSO used in this study cause cell damage. As shown in Figure 6, no significant adverse effect was observed on the viability of PC12 cells after cells were treated only with ZnPP or BSO (cell viability: 99.34% ± 2.60% and 98.07% ± 0.80%, respectively). In the groups without