5. RNA-seq差异表达基因GO和KEGG富集分析表明，50 mM

GO gene and KEGG 5. RNA-seq enrichment analysis showed differential expression, 50 mM NaCl under treatment, in the molecule functional classification, leaves significant enrichment of differentially expressed genes "active transition metal ion transport membrane", "transport activities" and "iron transmembrane transport activity" stage; root significant enrichment of differentially expressed genes "ionic bonding", "substrate specificity transmembrane transporter activity" and "metal ion binding" and other functions. In biological processes, leaves significant enrichment of differentially expressed genes "ion transport", "oxidative stress" and "iron ion reaction" stage, roots significant enrichment of differentially expressed genes "transport membrane", " stimulation "and" redox processes "stage. Description of the Related genes involved in the above processes play a major role in the response to King Processing salt. Further, it was found related to the plant hormone signal transduction abiotic stress response, phenylpropanoid biosynthesis and biosynthesis of secondary metabolites significantly enriched. Phenylpropanoid biosynthesis especially in the salt treatment and the root of the blade in each stage are significant enrichment, in particular 24 h, the salt enriched more significant, indicating phenylpropanoid secondary metabolites and the like in response to King also plays a salt treatment Key role.

5. RNA-seq differential expression gene GO and KEGG enrichment analysis showed that under the processing of 50 mM NaCl, the leaf differential gene was significantly enriched in the process of "transition metal ion transmembrane transport activity", "transport activity" and "iron ion transmembrane transport activity"; Ion binding, substrate-specific transmembrane protein activity, and metal ion binding. In the biological process, the leaf differential gene is significantly concentrated in the processes of "ion transport", "oxidation stress response" and "reaction to iron ion", and the gene of difference in root is significantly enriched in the process of "cross-membrane transport", "stimulation reaction" and "oxidation reduction process". It is explained that the relevant genes involved in the above process play a major role in the overlord response salt treatment. In addition, significant enrichment of plant hormone signal transduction associated with abiotic stress response, biosynthesis of secondary metabolites and biosynthesis of phenylpropyl swashes was found. In particular, phenylpropyl biosynthesis is significantly enriched at roots and leaves at all stages of salt treatment, especially salt treatment 24 h enrichment is more significant, indicating that secondary metabolites such as phenylpropylene also play a key role in overlord response to salt treatment.

5. The enrichment analysis of RNA SEQ differentially expressed genes go and KEGG showed that under the treatment of 50 mM NaCl, the leaf differentially expressed genes were significantly enriched in the processes of "transition metal ion transmembrane transport activity", "transport activity" and "iron ion transmembrane transport activity"; the root differentially expressed genes were significantly enriched in the processes of "ion binding", "substrate specific transmembrane transport protein activity" ”And "metal ion combination" and other functions. In the biological process, the difference genes in leaves were significantly enriched in the processes of "ion transport", "oxidative stress response" and "response to iron ions", while the difference genes in roots were significantly enriched in the processes of "transmembrane transport", "stimulus response" and "redox process". It is suggested that the genes involved in the above processes play an important role in the response of overlord to salt. In addition, it was found that abiotic stress related plant hormone signal transduction, secondary metabolite biosynthesis and phenylpropanoid biosynthesis were significantly enriched. In particular, phenylpropanoid biosynthesis was significantly enriched in roots and leaves at all stages of salt treatment, especially in 24 hours of salt treatment, indicating that secondary metabolites such as phenylpropanoid also played a key role in response to salt treatment.