The PCR instrument recommended for the detection of ruminant species DNA in crude heparin (FDA industrial Guideline Method) is smartcycle, and the method is transferred to cfx96 touchtm PCR instrument. Due to the differences in temperature control mode, software analysis and data processing mode, when the same amplification kit is applied to different brands / models of instruments, it shows certain differences: when the DNA of ruminant / pig species with high concentration is amplified, Cy5 lags on the two instruments, On cfx96 touchtm, the instrument does not report amplification or the reported detection value is > 37; Amplification is not reported on smartcycle.
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