Figure 3. A comparison of EvaGreen® Dyes during melt curve analysis. EvaGreen® Plus Dye (Solid, Dark Green) and original EvaGreen® Dye (Dashed, Light Green), two replicates each. The raw fluorescence melt plot (A) reveals that EvaGreen® Plus Dye is brighter when bound to dsDNA and has a lower post-melt background fluorescence. The derivative melt plot (B) displays no significant shift to melt temperatures or DNA disassociation characteristics