针对茶多酚的溶剂提取方法，本课题利用有机溶剂萃取法用乙醇水溶液代替蒸馏

针对茶多酚的溶剂提取方法，本课题利用有机溶剂萃取法用乙醇水溶液代替蒸馏水提取分离茶多酚粗品，用乙酸乙酯代替有毒溶剂氯仿对提取的粗品进行进一步的纯化。选择不同的温度、溶剂浓度、料液比和加热时间，以及震荡和静置时间，对茶叶中茶多酚的影响。通过测定紫外吸光度，计算得到茶多酚的浸出率，得到最佳工艺条件寻找最佳工艺条件，以获得最大的收率。结果表明，主次因素先后顺序为：C>D>A>B，A2B1C2D2，，即乙醇浓度为60%、料液比为1：40、浸提温度为70℃、浸提时间为50 min；萃取震荡时间为8 min，萃取静止时间为70 min。

针对茶多酚的溶剂提取方法，本课题利用有机溶剂萃取法用乙醇水溶液代替蒸馏水提取分离茶多酚粗品，用乙酸乙酯代替有毒溶剂氯仿对提取的粗品进行进一步的纯化。 选择不同的温度、溶剂浓度、料液比和加热时间，以及震荡和静置时间，对茶叶中茶多酚的影响。 通过测定紫外吸光度，计算得到茶多酚的浸出率，得到最佳工艺条件寻找最佳工艺条件，以获得最大的收率。 结果表明，主次因素先后顺序为：C>D>A>B，A2B1C2D2，，即乙醇浓度为60%、料液比为1：40、浸提温度为70℃、浸提时间为50 min；萃取震荡时间为8 min，萃取静止时间为70 min。

In view of the solvent extraction method of tea polyphenols, this project uses the organic solvent extraction method to extract and separate the crude tea polyphenols with ethanol aqueous solution instead of distilled water, and further purify the crude tea polyphenols with ethyl acetate instead of the toxic solvent chloroform. The effects of different temperature, solvent concentration, solid-liquid ratio, heating time, shaking and standing time on tea polyphenols were studied. Through the determination of UV absorbance, the extraction rate of tea polyphenols was calculated, and the optimal process conditions were obtained, so as to obtain the maximum yield. The results showed that the order of the primary and secondary factors was as follows: C > d > a > b, a2b1c2d2, that is, the ethanol concentration was 60%, the solid-liquid ratio was 1:40, the extraction temperature was 70 ℃, the extraction time was 50 min, the extraction vibration time was 8 min, and the extraction static time was 70 min.<BR>