Decidual tissue was washed, minced,

Decidual tissue was washed, minced, and digested with 0.1 mg/mL collagenase IV and 0.01 mg/mL DNase I (Sigma) shaking in a water bath for 1 h at 37 °C. Released lymphocytes were filtered through 100-, 70-, and 40-μm sieves (BD Labware). Lymphocytes were dissolved in 20 mL 1.023g/mL Percoll (GE Healthcare) and layered on 10 mL 1.080g/mL and 12.5 mL 1.053g/mL Percoll for density gradient centrifugation (30 min at 800 × g). Lymphocytes were isolated from the 1.080 to 1.053 g/mL interface and dMϕ from the 1.053 to 1.023 g/mL interface. Cells were washed and directly stained for flow cytometric analysis or FACS sort (SI Appendix, Fig. S8). pNK, pT, and monocytes from leucopacks were isolated with RosetteSep (StemCell Technologies). For all leukocyte types, >95% purity was obtained.Cocultures, RNA isolation, microarray hybridization and analysis, flow cytometry, cytokine analysis, and imaging and statistics used are described in SI Appendix.