微生物酶作为生物催化剂, 与化学催化剂相 比, 具有更高的催化效率、更

Enzyme as a biocatalyst a microorganism, as compared to chemical catalysts, having a higher catalytic efficiency, higher reaction specificity and stereoselectivity and mild reaction conditions. Resolving enzyme in active chiral center having the characteristics, the reaction rate of enantiomers has the ability to identify active enantiomers by an enzyme which is higher than the matching characteristics of mismatched diastereomers, enantiomers separated . At present, because enzymes and microorganisms capable of catalyzing the asymmetric hydrolysis of the ester, and esterification and asymmetric alcohols, scholars mainly two ways to take advantage of this split d l- menthol. <br>Often in the non-aqueous phase, the organic phase reaction medium separation, reverse micelles of ionic liquids and the like. Acyl donor is used with a carboxylic acid, anhydride, alkenyl esters and the like. Wen-Hsin Wu et using Candida lipase AY 30 to a butyric anhydride acyl donor transesterification split ee% of the product obtained is 86%, E is 4.Dong-Ling Wang et al., the use of immobilized lipase for the resolution dl- menthol, eep%> 95%, E value is 92. Others oxime as acyl donor, using Pseudomonas lipase resolution of menthol, which is a value up to 98% eep% [6].

微生物酶作为生物催化剂, 与化学催化剂相 比, 具有更高的催化效率、更高的反应专一性和立 体选择性以及温和的反应条件 。拆分时酶的活性 中心具有手性特征, 对对映体有识别能力, 利用其 与酶的活性中心匹配的对映体反应速度高于不匹 配的对映体的特征, 将对映体分离。目前由于酶和 微生物能催化酯和醇的不对称水解和不对称酯化, 国内外学者主要利用这两种途径来拆分 d l-薄荷醇 。<br>往往在非水相中，其反应介质分有机相、离子液体以及反胶束体系等。所采用的酰基供体则有羧酸、酸酐、烯酯等。Wen-Hsin Wu等采用假丝酵母脂肪酶AY一30以丁酸酐为酰基供体进行转酯化拆分，得到的产物为e.e.%值为86%,E值为4.Dong-Ling Wang等,则采用固定化脂肪酶对dl-薄荷醇进行了拆分,e.e.p%>95%，E值为92。还有人以肟为酰基供体，采用假单胞菌脂肪酶对薄荷醇进行了拆分，其e.e.p%值可达98%[6]。

As a biocatalyst, microbial enzyme has higher catalytic efficiency, higher reaction specificity, stereoselectivity and mild reaction conditions than chemical catalyst. At the time of resolution, the active center of the enzyme has the chirality characteristic, and the enantiomer has the recognition ability. The enantiomer can be separated by using the characteristic that the reaction speed of the enantiomer matched with the active center of the enzyme is higher than that of the unmatched enantiomer. At present, because enzyme and microorganism can catalyze asymmetric hydrolysis and esterification of ester and alcohol, scholars at home and abroad mainly use these two ways to resolve d l menthol.<br>In the non-aqueous phase, the reaction medium consists of organic phase, ionic liquid and reverse micelle system. The acyl donors used are carboxylic acid, anhydrides, alkenyl esters, etc. Wen Hsin Wu et al. Used Candida lipase ay-30 to transesterify and separate with succinic anhydride as acyl donor. The products obtained were E.E.% value 86%, e value 4.dong-ling Wang et al. Then the DL menthol was separated with immobilized lipase, E.E.P% > 95%, e value 92. Others used oxime as acyl donor and Pseudomonas lipase to separate menthol. The value of E.E.P% was 98% [6].<br>